Figure 3.

(a–d) Immunostaining for Fasn, and (e–g) cytokeratin 19 in (a and e) control and irinotecan group (b, d, f and g) on day 4, and the number of (h) Fasn-strongly-positive hepatocytes. (b) An arrow and (d) a closed arrowhead indicate cells accompanying two nuclei, and (f) open arrowheads show cytokeratin 19-positive cells forming clusters. The data shown are from a representative preparation set and are similar to results obtained in two other sets. (a–c) Scale bars 100 μm, and (d–g) 25 μm. (h) The number of Fasn-strongly-positive hepatocytes. Open bars indicate the number around the CV and closed bars around the BD. Hatched bars represent the number of cells with two nuclei. Data are the mean ± SD from 3 rats. These cells were quantified from five microscope fields (0.14 mm²/field) for each rat. ^**p<0.01 vs. control (two-tailed Student’s t-test). ^†p<0.05 and ^††p<0.01 between the values for CV and the BD (two-tailed Student’s t-test). Fasn, fatty acid synthase; CV, central vein; BD, bile ducts.