Table III.
The mRNA by RT-PCR evaluation for cholesterol and fatty acid metabolism in control and irinotecan group.
| Cellular function and gene name | Gene symbol | Signal
|
|||
|---|---|---|---|---|---|
| Control | Day 4 | Day 18 | Day 25 | ||
| Cholesterol synthesis | |||||
| HMG-CoA reductase | Hmgcr | 1 | 10.14 | 1.07 | 1.97 |
| Isopentenyl-diphosphate Δ-isomerase 1 | Idi1 | 1 | 9.9 | 1.07 | 0.92 |
| Squalene epoxidase | Sqle | 1 | 55.19 | 12.54 | 6.7 |
| Bile acid synthesis | |||||
| Cytochrome P450, family 7, subfamily a, polypeptide 1 | Cyp7a1 | 1 | 7.73 | 1.91 | 0.59 |
| Cholesterol and bile acid export | |||||
| ATP-binding cassette, sub-family G member 5 | Abcg5 | 1 | 1.17 | 1.88 | 0.55 |
| ATP-binding cassette, sub-family B (MDR/TAP), member 11 | Abcb11 | 1 | 1.07 | 1.02 | 1.11 |
| Solute carrier family 10 (sodium/bile acid co-transporter family), member 1 | Slc10a1 | 1 | 1.24 | 0.87 | 1.84 |
| Bile acid conjugation | |||||
| UDP glucuronosyltransferase 2 family, polypeptide B | Ugt2b | 1 | 7,362 | 3,310 | 2,392 |
| Fatty acid synthesis | |||||
| Fatty acid synthase | Fasn | 1 | 3.58 | 3.85 | 0.81 |
| Acyl-CoA synthetase short-chain family member 2 | Acss2 | 1 | 12.11 | 6.9 | 2.14 |
| Acyl-CoA synthetase medium-chain family member 5 | Acsm5 | 1 | 11.94 | 22.49 | 6.03 |
| Acyl-CoA synthetase long-chain family member 5 | Acsl5 | 1 | 5.53 | 6.74 | 1.99 |
| Elongation of long chain fatty acid member 6 | Elovl6 | 1 | 20.23 | 1.19 | 0.55 |
| Transcription factor | |||||
| Early growth response 1 | Egr1 | 1 | 3.55 | 23.32 | 6.16 |
| Kupffer cells | |||||
| Chemokine (C-X-C motif) ligand 9 | Cxcl9 | 1 | 0.2 | 0.34 | 0.51 |
| Monocytes | |||||
| Ly6-C antigen | Ly6c | 1 | 0.55 | 1.26 | 1.62 |
mRNA levels in the irinotecan group are expressed relative to the values of individual mRNAs in the control group.