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(A and B) Flow cytometric analysis of the cell cycle in Daoy and Saos-2 cells following MTX treatment. Cells were analyzed at day 3 and 6 of incubation with MTX. (C) The percentage of the sub-G1 population was evaluated at the same time points. x-axis, doses of MTX and days of treatment (A–C). y-axis, percentage of cells in specific phases of the cell cycle (A and B); percentage of cells in sub-G1 phase (C). MTX, methotrexate.
