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. 2015 Apr 2;12:64. doi: 10.1186/s12974-015-0283-y

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© Li et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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NRG-1 reduces DFP-induced expression of pro-inflammatory cytokine mRNA in the brain. RNA was isolated from the hippocampus and analyzed by RT-PCR to quantify the expression of mRNA for IL-1β (A), IL-6 (B), and TNFα (C) (n = 7 to 9 biological replicates for each condition). The data are presented as mean ± SEM of the relative mRNA (in arbitrary values) levels in naive, control, DFP, and DFP + NRG-1-treated rats after normalization to GAPDH mRNA levels (**P < 0.01; ***P < 0.001; ****P < 0.0001).