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. Author manuscript; available in PMC: 2015 Apr 9.
Published in final edited form as: Stem Cells. 2009 Oct;27(10):2383–2392. doi: 10.1002/stem.185

Figure 4. STAT3 inhibitor treatment causes increased apoptosis in serum-derived GBM cell lines, but not in GBM stem cells.

Figure 4

A. GS7-2 and GS6-22 cells were treated with STAT3 inhibitor or DMSO control for 3 days, stained with annexinV-FITC, and analyzed via flow cytometry. H2O2 treatment (4.4 mM) was performed for 4 hours as a positive control. Cells were stained using the AnnexinV-FITC Apoptosis Detection Kit (BD Biosciences) according to the manufacturer's instructions. Values represent mean of three samples; bars SE. B. The A172 serum-derived glioma cell line was treated for 3 days with STA-21 or DMSO and analyzed as described above. Values represent mean of three experiments; bars SE.