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. 2015 Apr 10;46(1):38. doi: 10.1186/s13567-015-0175-2

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© Kerner et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of recombinant Shiga toxins and toxoids on CD77 expression by bovine CD8β ⁺ PBMC. Cells were incubated in culture medium containing 5 μg/mL PHA-P and rStx_WT or rStx_mut as indicated. After four days of incubation cells were submitted to immunolabelling and flow cytometry analysis. Percentages of CD8β⁺ PBMC co-expressing CD77 (events in the upper right quadrant) are given in the upper right corner of the dot plots. PBMC incubated in the presence of PHA-P and vector control were used as control (upper graph).