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. 2015 Apr 10;46(1):38. doi: 10.1186/s13567-015-0175-2

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© Kerner et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Gene transcription in bovine iIEL and surface marker expression on bovine MDM incubated for 6 h with rStx _WT or rStx _mut . (A) Relative amounts of gene transcripts for IL-4 harboured by PHA-P-stimulated (2.5 mg/mL) bovine iIEL normalized to the transcription of the housekeeping gene GAPDH. Vector control cultures were used as reference and set to 100% (dashed line). (B) Expression (i.e., mean fluorescence intensity; MFI) of CD14 on bovine CD77⁺ MDM relative to vector control cultures defined as 1.0 (dashed line). Data is depicted as means ± standard deviations from 5 independent experiments each. Statistical analysis was performed as described in legend to Figure 2. Significance levels were defined as p ≤ 0.01 [**], and p ≤ 0.05 [*].