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. 2015 Apr 10;46(1):38. doi: 10.1186/s13567-015-0175-2

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© Kerner et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of Stx1 and Stx2 neutralizing antibodies (nAb) in sera from two calves vaccinated with rStx1 _mut and rStx2 _mut . Results from the Vero cell neutralization assay (VNA). Dashed line indicates the detection limit. Calves were vaccinated on days 0 and 21 of the trial. A titre of 30 was attributed to all those samples that gave negative test results in the highest concentration tested.