Fig 2. cGMP responses during Trypanosoma cruzi trypomastigotes adhesion to extracellular matrix.

Trypomastigotes (1x10⁹) were incubated with ECM (1.5 mg) in phenol red free-MEM, supplemented with 2% FBS, for 2 h at 37°C and 5% CO₂. cGMP levels were measured in T. cruzi extracts by competitive ELISA. The asterisk represents a p<0.001 according to t-Student test. Values are the mean of four independent experiments.