Table 1. Putative Tyrosine nitrated T. cruzi proteins identified by nLC/MS-MS after sample enrichment by immunoprecipitation.
| Group | Protein | Gene ID | Function | Theoretical | |
|---|---|---|---|---|---|
| MM (Da) | pI | ||||
| Ty | 10 kDa heat shock protein, putative | TcCLB.508209.90 | Protein folding | 10701 | 9.07 |
| Ty | 40S ribosomal protein S10, putative | TcCLB.506679.140 | Translation | 18250 | 10.8 |
| Ty | 40S ribosomal protein S14, putative | TcCLB.508823.50 | Translation | 15568 | 10.6 |
| Ty | 40S ribosomal protein S24E, putative | TcCLB.507681.150 | Translation | 15668 | 11.7 |
| Ty | 40S ribosomal protein S3a-1 OS | TcCLB.510999.39 | Translation | 28288 | 11 |
| Ty | 40S ribosomal protein S4, putative | TcCLB.509683.117 | Translation | 30876 | 11.1 |
| Ty | 40S ribosomal protein S6, putative | TcCLB.507709.50 | Translation | 21400 | 11.4 |
| Ty | 60S ribosomal protein L10a, putative | TcCLB.506963.10 | Translation | 25146 | 10.2 |
| Ty | 60S ribosomal protein L13, putative | TcCLB.508153.280 | Translation | 25294 | 11.9 |
| Ty | 60S ribosomal protein L18, putative | TcCLB.506181.50 | Translation | 34028 | 11.1 |
| Ty | 60S ribosomal protein L23a, putative | TcCLB.508175.146 | Translation | 21268 | 11.5 |
| Ty | 60S ribosomal protein L28, putative | TcCLB.510101.30 | Translation | 16368 | 12.2 |
| Ty | 60S ribosomal protein L6, putative | TcCLB.507709.50 | Translation | 21400 | 11.4 |
| Ty | 60S ribosomal protein L7a, putative | TcCLB.510835.40 | Translation | 34550 | 11.8 |
| Ty, Ty+ECM | ADP, ATP Carrier protein 1, mitocondrial precursos, putative | TcCLB.506211.160 | Transport | 34955 | 10.3 |
| Ty, Ty+ECM | alpha tubulin, putative | TcCLB.411235.9 | Cytoskeleton | 49800 | 4.7 |
| Ty | ATP synthase, epsilon chain, putative | TcCLB.506945.240 | Metabolism | 20246 | 6.08 |
| Ty, Ty+ECM | beta tubulin, putative | TcCLB.506563.40 | Cytoskeleton | 49701 | 4.43 |
| Ty | beta-fructofuranosidase-like protein | TcCLB.506705.70 | Metabolism | 53584 | 7.79 |
| Ty | chaperonin HSP60, mitochondrial precursor | TcCLB.510187.551 | Protein folding | 30553 | 9.15 |
| Ty, Ty+ECM | cytochrome c, putative | TcCLB.506949.50 | Metabolism | 12234 | 9.88 |
| Ty, Ty+ECM | elongation factor 1-alpha, putative | TcCLB.511369.30 | Translation | 30736 | 9.33 |
| Ty | enoyl-CoA hydratase, mitochondrial precursor, putative | TcCLB.508185.10 | Metabolism | 28818 | 8.91 |
| Ty, Ty+ECM | glyceraldehyde 3-phosphate dehydrogenase, putative | TcCLB.506943.50 | Metabolism | 39033 | 9.2 |
| Ty, Ty+ECM | glycosomal malate dehydrogenase, putative | TcCLB.506503.69 | Metabolism | 34068 | 8.88 |
| Ty | guanosine monophosphate reductase, putative | TcCLB.506519.130 | Metabolism | 52311 | 9.05 |
| Ty, Ty+ECM | heat shock 70 kDa protein, putative | TcCLB.511211.170 | Protein folding | 73299 | 5.22 |
| Ty, Ty+ECM | histone H2A, putative | TcCLB.510525.80 | DNA packaging | 14354 | 11.9 |
| Ty, Ty+ECM | histone H2B, putative | TcCLB.511635.10 | DNA packaging | 12361 | 12.2 |
| Ty, Ty+ECM | histone H3, putative | TcCLB.505931.50 | DNA packaging | 14762 | 11.9 |
| Ty, Ty+ECM | histone H4, putative | TcCLB.510351.20 | DNA packaging | 11170 | 11.7 |
| Ty+ECM | hypothetical protein, conserved (pseudogene) | TcCLB.511821.179 | N/A | 143829 | 4.19 |
| Ty | hypothetical protein, conserved | TcCLB.504089.70 | N/A | 12959 | 8.48 |
| Ty | hypothetical protein, conserved | TcCLB.504001.10 | N/A | 13465 | 9.78 |
| Ty+ECM | hypothetical protein, conserved | TcCLB.510877.40 | N/A | 24454 | 10.2 |
| Ty | hypothetical protein, conserved | TcCLB.510143.5 | N/A | 7311 | 6.52 |
| Ty | hypothetical protein, conserved | TcCLB.508719.70 | N/A | 44107 | 10.6 |
| Ty, Ty+ECM | kinetoplast DNA-associated protein, putative | TcCLB.508719.60 | DNA packaging | 14361 | 12.1 |
| Ty | kinetoplast-associated protein 3 | TcCLB.509791.120 | DNA packaging | 21245 | 11.9 |
| Ty | mitochondrial oligo_U binding protein TBRGG1, putative | TcCLB.507927.20 | RNA processing | 97266 | 8.8 |
| Ty | poly(A)-binding protein, putative | TcCLB.508461.140 | RNA processing | 61411 | 9.69 |
| Ty | ribosomal protein l35a, putative | TcCLB.506559.470 | Translation | 16892 | 12.1 |
| Ty | ribosomal protein L36, putative | TcCLB.509671.64 | Translation | 12948 | 12.1 |
| Ty, Ty+ECM | ribosomal protein S25, putative | TcCLB.504105.94 | Translation | 12351 | 11.2 |
| Ty | ribosomal protein S7, putative | TcCLB.506593.19 | Translation | 23915 | 11.9 |
| Ty | RNA-binding protein, putative | TcCLB.508413.50 | RNA Processing | 31097 | 9.78 |
Tyrosine nitrated T. cruzi proteins after 2 h incubation of the parasite in the presence of bovine serum albumin (Ty) or extracellular matrix (Ty+ECM).