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Primer sequences and expected amplicon sizes for the gene amplification.
| cDNA | Genbank Acc. number | Forward primer | Reverse primer | Amplicon size (bp) |
|---|---|---|---|---|
| rOat1 | NM017224 | CATTGATGGC TGGGTCTATG |
CAAGGTTCCACT CAGTCACG |
65 |
| rOat3 | NM031332 | ATCTCATCAACA TCTATTGGGTACTG |
CAGAGAGAGACA GAAGGTCACAC |
371 |
| β-actin | NM031144 | ATGGTGGGTA TGGGTCAGAA |
GGGGTGTTGAAG GTCTCAAA |
241 |
rOat1: rat organic anion transporter 1; rOat3: rat organic anion transporter 3.
