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. 2015 Apr 1;14:44. doi: 10.1186/s12934-015-0224-6

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© van Dissel et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Morphology of S. lividans 66 and its derivatives PM01 and PM02 in submerged culture. PM01 was obtained after 70 generations in a nitrogen-limited chemostat at a dilution rate of 0.1 h^-1. PM02 was obtained after evolving PM01 for another 30 generations in a phosphate-limited chemostat at 36°C and a dilution rate of 0.1 h^-1. Top row: growth of strains in a baffled shake flask for 30 h on TSBS. Bottom row: cultivation of strains in a 900 ml bioreactor in TSBS media after 24 h of growth. Scale bar, 200 μm.