Fig 5. Induction of VLP-specific CD4⁺ and CD8⁺ T-cell responses in Ad-EVVLP-immunized mice.

Splenocytes from individual mice immunized twice s.c. with PBS, Ad-LacZ, Ad-EVVLP, or FI-EV71 were cultured in the presence or absence of 10⁷ pfu UV-EV71 5746 for 48 h. (A) CD4⁺ T cell proliferation in response to EV71 particles was analyzed using flow cytometry with PE-Cy5-conjugated anti-CD4 antibodies. (B) Splenocytes were stained with PE-Cy5-conjugated anti-CD8 antibodies and subsequently fixed and stained with PE-conjugated anti-IFN-ɣ antibodies, and analyzed using flow cytometry. The results were presented as the mean of the percentage of CD4⁺ or CD8⁺ T cells after antigen stimulation compared to the gated CD4⁺ T cells without antigen stimulation that were set as 0%. Five mice per group were assayed.