Table 1. Induction of neutralizing antibodies against EV71 E59 and 5746 strains and CVA16 by Ad-EVVLP or FI-EV71 vaccine.

	Route	Strain	EV71-specific neutralizing antibody titers (mean)
Ad-LacZ	i.p/s.c/oral	E59/5746/CVA16	<1:8
Ad-EVVLP	i.p.	E59	1:64
		5746	1:128
		CVA16	<1:8
	s.c.	E59	1:64
		5746	1:64
		CVA16	<1:8
	oral	E59	<1:8
		5746	<1:8
FI-EV71 (0.1 μg)	s.c.	E59	1:256
FI-EV71 (1 μg)	s.c.	5746	1:512
		CVA16	<1:8

Seven-week-old BALB/c mice were individually primed and boosted at 14-day intervals i.p., s.c., or orally with 10⁸ pfu Ad-EVVLP or Ad-LacZ. Sera collected on Day 21 were analyzed for neutralizing activity by incubating 10² pfu 5746, E59 or CVA16 with varying dilutions of individual immune sera before being added to RD cells. CPE was observed after 5 days of culture. The results were expressed as neutralizing titers that correspond to the dilution of immune sera, giving TCID₅₀ value of 50% reduction of cytopathic effect.