Fig 1. Immunoblotting to evaluate antibody binding to B. turicatae protein lysates, rGlpQ, and rBipA (A).

The asterisk and arrowhead represent the molecular masses for native GlpQ and BipA, respectively (A). rBipA was produced as a thioredoxin fusion protein and is 15 kDa larger than the native protein. Immunoblots were also probed with a serum sample from an uninfected patient (B) and a monoclonal antibody against the six-histidine fusion tag (C and D). Molecular masses are shown to the left of each immunoblot.