. Author manuscript; available in PMC: 2015 Apr 9.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jul 4;934:16–21. doi: 10.1016/j.jchromb.2013.06.031

Table 2.

% Recovery and extraction reproducibility. Comparison of spike then precipitate vs. precipitate then spike. % CVs are for precipitate then spike (n = 5).

	2 ng/ml	10 ng/ml	20 ng/ml	500 ng/ml
Testosterone	66.7	61.1	58.0	N/A
% CV	25.2	25.7	24.6	N/A
Dihydrotestosterone	59.7	57.5	56.8	N/A
% CV	13.6	25.4	17.0	N/A
Progesterone	66.0	63.6	60.8	N/A
% CV	19.4	20.5	22.0	N/A
Cortisone	50.6	60.7	54.5	96.4
% CV	17.9	22.2	16.3	25.9
Cortisol	19.7	58.0	53.3	90.3
% CV	32.8	32.6	35.3	26.5

% CV for spike then precipitate: extraction reproducibility.