Phosphorylation protein level of p53, chk-2, and p27 in control, HPAH, and IPAH HPASMC. The human phospho-antibody array detected phosphorylated proteins in cell lysates from one donor control, one HPAH, and one IPAH HPASMC. Phosphorylation profiles were created by quantifying the mean spot pixel densities normalized by positive control dots. Array signals from scanned X-ray film images were analyzed using image analysis software NIH ImageJ. The intensity of the dots were normalized by the positive control. The statistical analysis was performed using t-test. *P < 0.05 compared with control.