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. 2015 Apr 9;10(4):e0111108. doi: 10.1371/journal.pone.0111108

Fig 4. Phenotypes of M. oryzae Δmet6 mutants in axenic cultures.

Fig 4

A, M. oryzae wild type (P1.2) and Δmet6 mutants (M15.1; M22.1 and M23.1) were grown on minimal medium (MM) with sulfate as sole sulfur source (left) or in MM supplemented with 1 mM methionine (MM + Met, right). Δmet6 mutants were unable to grow on MM, but grew normally on MM + Met. B, M. oryzae wild type (P1.2) and Δmet6 mutant (M23.1) were grown on rice agar medium (RM) in the absence or presence of 1 mM Met (RM + Met). Δmet6 mutant M23.1 displayed a radial growth similar to WT on RM, but developped a brown-orange color differing from the grey color of WT. Addition of methionine to MM rescued the color phenotype of Δmet6.