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. 2015 Apr 9;10(4):e0111108. doi: 10.1371/journal.pone.0111108

Fig 5. Pathogenicity of M. oryzae Δmet6 mutants on barley and rice.

Fig 5

Left, Detached Barley leaves (cv. Express) were inoculated with droplets of spore suspension of M. oryzae wild type P1.2 (1) and Δmet6 mutant M23.1 in the presence (2) or absence (3) of 1 mM methionine. Δmet6 mutant M23.1 was non-pathogenic on detached barley leaves (no lesion). Addition of 1 mM methionine to Δmet6 spores rescued its pathogenicity defect. Right, Young rice plants (cv. Sariceltik, 4 leaves stage) were spray inoculated with spore suspensions of wild type P1.2 (1), three Δmet6 mutants (2: M15.1; 3: M22.1; 4: M23.1) and a transformant corresponding to the complementation of Δmet6 mutant (M15.1) with MET6 wild type allele (5). Δmet6 mutants M15.1, M22.1 and M23.1 were non-pathogenic on rice leaves (no lesion). Complementation of Δmet6 mutants M15.1 with wild type MET6 allele rescued its pathogenicity defect.