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. 2015 Apr 9;10(4):e0111108. doi: 10.1371/journal.pone.0111108

Fig 6. Expression profiles of MET6 and genes from reversed transulfuration pathway in M. oryzae Δmet6 mutants and wild type.

Fig 6

A, Transcript levels in mycelium of wild type P1.2 isolate grown in MM (MM: black bar) or in MM supplemented with 1 mM methionine (MM-Met: grey bar). Addition of methionine to MM down-regulated MET6 (2 fold) and up-regulated CGL1 fro reverse transsulfuration pathway (1.7 fold). B, Transcript levels in mycelium of wild type P1.2 (grey bar) and three Δmet6 mutants (M15.1: punctuated grey bar; M22.1: left shaded grey bar; and M23.1: right shaded grey bar) grown on MM supplemented with 1 mM methionine. CBS1 and CGL1 are both up-regulated in Δmet6 mutants. Gene expression was normalized relative to the level of the constitutive gene ILV5 (MGG_15774.7). Each bar is the mean value of three replicates with standard deviations as vertical lines. Analyzed genes were: MET6, methionine synthase; CBS1, cystathionine β-synthase; CGL1, cystathionine γ-lyase. *Differences in expression are significant according to a t-test (5%).