Table 1. Nutrient requirements of M. oryzae Δmet6mutants.
| Growth medium | Wild type | Δmet6 |
|---|---|---|
| Minimal medium (MM) | +++ | - |
| MM + Cysteine, 1mM | +++ | - |
| MM + Glutathione, 1mM | +++ | - |
| MM + Cystathionine, 1 mM | +++ | - |
| MM + Homocysteine, 1 mM | - | - |
| MM + Homocysteine, 0.1 mM | ++ | - |
| MM + Methionine, 1 mM | +++ | +++ |
| MM + S-adenosylmethionine (SAM), 1 mM | +++ | + |
| MM + S-methylmethionine (SMM), 1 mM | +++ | + |
| MM + Methylthioadenosine (MTA), 1 mM | +++ | + |
| MM + Betaine, 1 mM | +++ | - |
| MM + Choline, 1 mM | +++ | - |
Wild type (P1.2) and Δmet6 M. oryzae mutants (M15.1, M22.1, M23.1) were grown on minimal medium (MM) supplemented with different sulfur metabolites. Mycelium growth was observed 4 to 6 days after transfert of a mycelial plug from CM to MM. MTA was dissolved in dimethylsulfoxide (DMSO) and added to MM at 1% DMSO final concentration.
+++, normal growth
++, reduced growth
+, highly reduced growth
-, no growth.