Table 3. Effects of exogenous methionine on sulfur amino acids and glutathione levels in M. oryzae Δmet6 mutants and wild type.

*nmoles.mg-1 dry weight	GSH	Cys	CTT	Hcys	Met
Wild type P1.2, MM	*11 ± 2	0.2 ± 0.08	1.5 ± 0.4	0.06 ± 0.04	1.5 ± 0.4
Wild type P1.2, MM + Met	9.3 ± 2	0.1 ± 0.06	4.2 ± 1.4	0.1 ± 0.05	1.3 ± 0.4
Fold change ** P12-Met/P12	0.85	0.5	2.8 °	1.6 °	0.85
Δmet6 M15.1, MM + Met	9.5 ± 2	0.3 ± 0.16	41 ± 6.7	1.9 ± 0.4	1.5 ± 0.7
Δmet6 M22.1, MM + Met	8.9 ± 2	0.3 ± 0.15	38.5 ± 3	1.9 ± 0.8	1.0 ± 0.3
Δmet6 M23.1, MM + Met	8.7 ± 2	0.2 ± 0.02	38 ± 4.0	2.2 ± 0.7	1.2 ± 0.2
Fold change ** Δmet6 / P1.2	0.95	2.6 °	9.3 °	20 °	0.95

Wild type M. oryzae isolate P1.2 was grown in liquid medium in absence (MM) or presence of 1 mM methionine (MM + Met) for 8 days. The Δmet6 mutants (M15.1, M22.1 and M23.1) were grown in liquid MM supplemented with 1 mM methionine (MM + Met) for 8 days. Cys (cysteine), CTT (cystathionine) and Hcys (homocysteine) were quantified by HLPC as described in Materials and Methods. Data were expressed in nmoles.mg^-1 dry weight and represent the mean (± standard deviation) of two technical replicates derived from three biological replicates.

**Fold change (Δmet6 / P1.2 MM + Met) was calculated using average value of the three mutant lines over wild type.

°Differences are significant according to a t-test (5%).