Table 4. Effects of exogenous methionine on methyl cycle metabolites levels in M. oryzae Δmet6 mutants and wild type.

*nmoles.mg-1 dry weight	SAM	SAH	SAM/SAH
Wild type P1.2, MM	*0.27 ± 0.1	0.07 ± 0.02	3.85
Wild type P1.2, MM + Met	0.35 ± 0.1	0.07 ± 0.02	4.85
Fold changes ** P12 + Met / P12	1.3 °	1	1.26
Δmet6 M15.1, MM + Met	1.25 ± 0.3	0.45 ± 0.16	2.7
Δmet6 M22.1, MM + Met	1.10 ± 0.17	0.30 ± 0.05	3.6
Δmet6 M23.1, MM + Met	0.75 ± 0.1	0.35 ± 0.06	2.2
Fold changes ** Δmet6 / P1.2	3.0 °	5.3 °	0.56

Wild type M. oryzae isolate P1.2 was grown in liquid medium in absence (MM) or presence of 1 mM methionine (MM + Met) for 8 days. Δmet6 mutants (M15.1, M22.1 and M23.1) were grown in liquid MM supplemented with 1 mM methionine (MM + Met) for 8 days. SAM and SAH were quantified by HPLC as described in Materials and Methods. Data were expressed in nmoles.mg^-1 dry weight and represent the mean ± standard deviation of 8 technical assays derived from three biological replicates.

Fold changes** (Δmet6 / P1.2 MM + Met) was calculated using average value of the three mutant lines over wild type.

°Differences are significant according to a t-test (5%).