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. 2015 Apr 9;10(4):e0123083. doi: 10.1371/journal.pone.0123083

Table 1. Stem-loop RT primers and PCR primers for 12 identified miRNAs.

Name Sequence(5’-3’)
miR156d stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgaggtgctctc
PCR prime Forward: acactccagctgggttgacagaag
miR156g stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgaggtgctctc
PCR prime Forward: acactccagctgggtgacagaaga
miR166b stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgagccccgagc
PCR prime Forward: acactccagctgggggaatgttgt
miR399a stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgagcagggcaa
PCR prime Forward: acactccagctgggtgccaaagga
miR319m stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgagagggagct
PCR prime Forward: acactccagctgggttggactgaa
miR160a stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgagtggcatac
PCR prime Forward: acactccagctgggtgcctggctc
miR164e stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgagtgcacgtg
PCR prime Forward: acactccagctgggtggagaagca
miR167c stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgagcagatcat
PCR prime Forward: acactccagctgggtgaagctgcc
miR169h stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgagcaggcaag
PCR prime Forward: acactccagctgggtagccaagga
miR162 stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgagtggacgca
PCR prime Forward: acactccagctgggtcgataaacc
miR5538 stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgaggcagcaag
PCR prime Forward: acactccagctgggactgaactca
miR4995 stem-loop RT-primer ctcaactggtgtcgtggagtccggcaattcagttgagcccttaac
PCR prime Forward: acactccagctgggaggcagtggc
miR# Universal reverse primer Reverse: aactggtgtcgtggag
5S rRNA PCR prime Forward: tctggtgtcctaggcgta
Reverse: attctggcgtcgagctat

#: 12 identified miRNAs.