Table 1. Stem-loop RT primers and PCR primers for 12 identified miRNAs.
Name | Sequence(5’-3’) | |
---|---|---|
miR156d | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgaggtgctctc |
PCR prime | Forward: acactccagctgggttgacagaag | |
miR156g | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgaggtgctctc |
PCR prime | Forward: acactccagctgggtgacagaaga | |
miR166b | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgagccccgagc |
PCR prime | Forward: acactccagctgggggaatgttgt | |
miR399a | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgagcagggcaa |
PCR prime | Forward: acactccagctgggtgccaaagga | |
miR319m | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgagagggagct |
PCR prime | Forward: acactccagctgggttggactgaa | |
miR160a | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgagtggcatac |
PCR prime | Forward: acactccagctgggtgcctggctc | |
miR164e | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgagtgcacgtg |
PCR prime | Forward: acactccagctgggtggagaagca | |
miR167c | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgagcagatcat |
PCR prime | Forward: acactccagctgggtgaagctgcc | |
miR169h | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgagcaggcaag |
PCR prime | Forward: acactccagctgggtagccaagga | |
miR162 | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgagtggacgca |
PCR prime | Forward: acactccagctgggtcgataaacc | |
miR5538 | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgaggcagcaag |
PCR prime | Forward: acactccagctgggactgaactca | |
miR4995 | stem-loop RT-primer | ctcaactggtgtcgtggagtccggcaattcagttgagcccttaac |
PCR prime | Forward: acactccagctgggaggcagtggc | |
miR# | Universal reverse primer | Reverse: aactggtgtcgtggag |
5S rRNA | PCR prime | Forward: tctggtgtcctaggcgta |
Reverse: attctggcgtcgagctat |
#: 12 identified miRNAs.