Fig 2. Targeted integration vector.

A. The vector created has several key features. These include an intact multicloning site and blue-white screening for cloning the genetic construct of interest, a dominant selectable marker (here NAT, but also constructed in NEO and HYG versions), bla, a bacterial selection marker, and 5’ and 3’ flanking regions that are homologous to the safe haven site in the genome. The polylinker sequence contains three rare cutting restriction enzyme recognition sites that are used to linearize the vector so that the 5’ and 3’ regions are subsequently flanking the construct. B. Representation of the selected safe haven site, and how the linearized vector inserts via homologous recombination.