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. 2015 Apr 9;10(4):e0123122. doi: 10.1371/journal.pone.0123122

Fig 2. EGCG and compound 30 inhibit FASN activity in the spinal cord of CCI-mice.

Fig 2

(A) The FASN activity was analyzed in the dorsal horn of the spinal cord of CCI-mice treated with vehicle, and 50 mg/Kg of EGCG, compound 23 (c23) and compound 30 (c30) as indicated in materials and methods at 14 and 56 days post injury (dpi). Data were expressed as a percentage with respect to vehicle-treated CCI-mice and represent the mean± SEM (n = 5). Data were analyzed by one-way ANOVA with Bonferroni’s post-hoc test. *p<0.05 and ***p<0.001 compared to vehicle-treated CCI-mice. (B) Protein extracts from the dorsal horn of the spinal cord of control mice (sham; Sh) and CCI-mice treated with vehicle (V), EGCG, compound 23 (c23) and compound 30 (c30) at 14 and 56 dpi were subjected to western blot to study the FASN protein levels. Representative immuno-blots showing no differences in FASN levels between all groups are presented. Actin levels were used as loading control.