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. 2015 Apr 9;10(4):e0123122. doi: 10.1371/journal.pone.0123122

Fig 3. Spinal cord of CCI-mice treated with EGCG and compound 30 at 14 dpi showed decreased levels of inflammatory cytokines.

Fig 3

(A) Total RNA of the dorsal horn of spinal cord of control mice (sham; Sh) and CCI-mice treated with vehicle (VEH) and 50 mg/Kg of EGCG, compound 23 (c23) and compound 30 (c30) was isolated at 14 and 56 days post injury (dpi) as indicated in materials and methods. Quantitative RT-PCR analysis of TNF-α, IL-1β and IL-6 was performed. Data were expressed as fold change normalized to 18S and represent the mean± SEM (n = 5). * p<0.05 and *** p<0.001 compared to vehicle-treated CCI-mice using a one-way ANOVA with Bonferroni’s post-hoc test. (B) Protein extracts from the dorsal horn of the spinal cord of control mice (sham; Sh) and CCI-mice treated with vehicle (VEH) and 50 mg/Kg of EGCG, compound 23 (c23) and compound 30 (c30) at 14 and 56 days post injury (dpi) were subjected to western blot to study the TNF-α, IL-1β and IL-6 protein levels. Data were expressed as a percentage with respect to sham-mice. Results are the mean± SEM (n = 5) and represent the ratio between each protein and actin levels, obtained by densitometric analysis of western blot. Data were analyzed by one-way ANOVA with Bonferroni’s post-hoc test. *** p<0.001 compared to sham mice and +++p<0.001 compared to vehicle-treated CCI-mice. (C) Representative immuno-blots are presented.