Table 1. Observed endpoints at specific time points during the zebrafish early life stage test.

	Developmental time point (hpf)
Observed endpoint	24	48	54	72	96
Coagulation	•	•	•	•	•
Tail detachment	•
Somite formation	•
Presence of heart beat	•	•	•	•	•
Hatching		•	*	*	•
Eye underdevelopment ¶	•	•
Pigmentation	•	•		•	•
Oedema pericard	•	•		•	*
Oedema yolk	•	•		•	•
Oedema head	•	•		•	•
Heart rate †	*	•
Malformation head	•	•		•	•
Malformation eye	•	•		•	•
Malformation mouth				•	•
Malformation otolith/sacculi	•	•		•	•
Malformation heart	•	•		•	•
Malformation pectoral fin					•
Malformation yolk	•	•		•	•
Malformation/irregular somites	•	•		•	•
Malformation tail	•	•		•	•
Curve in tail				•	•
Swim bladder inflation					*
Blood accumulation	•	•		•	•
Length †					*

Except for continuous variables, all endpoints were visually evaluated for presence or absence using a binary scoring system.

• Indicates at which time points each endpoint was observed.

* Indicates which endpoints were significantly affected by at least one of the knockdown conditions when compared to SCMO.

† Continuous variable

¶ Recognized by normal eye shape resembling younger embryos and/or by less iridiphores at certain timepoint.`

Hpf = hours post fertilization.