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. 2015 Mar 26;4:146. doi: 10.1186/s40064-015-0923-9

Table 1.

Use of the construct and event specific event assays to detect the FP967 TDNA

Norlin FP967 TDNA Hemizygote
Construct specific assay
P3 + P4 + probe2 X X
P1 + P2 + probe1 (detects SAD reference gene) X X X
Event specific assay
P13 + P14 + probe3 X X
P13 + P15 + probe 5 and P14 + P15 + probe 5 X X

The construct specific assay is able to distinguish between WT and transgenic plants, while the event specific assay can also distinguish between hemizygous and homozygous individuals. Sequences of the primers and probes are shown in Table 2, while their approximate location is shown on Figure 2.