Table 2.
Primer and probe sequences used in the construct and event specific promoters
| Primer/probe name | Sequence | Tm | Notes |
|---|---|---|---|
| SAD reference: Amplifies 68 bp of stearoyl-acyl carrier protein desaturase | |||
| P1 | GCTCAACCCAGTCACCACCT | 63 | |
| P2 | TGCGAGGAGATCTGGAGGAG | 61 | |
| prb1 | TGTTGAGGGAGCGTGTTGAAGGGA | 68 | |
| Construct specific assay: Used by commercial testing companies to detect 105 bp of FP967 TDNA | |||
| P3 | AGCGCGCAAACTAGGATAAA | 52 | In NOS 3’terminator |
| P4 | ACCTTCCGGCTCGATGTCTA | 55 | In DHFR gene of E.coli SpecR cassette |
| prb2 | CGCGCGCGGTGTCATCTATG | 67 | |
| Event Specific assay: Detects WT gDNA at scaffold261 or the FP967 TDNA RB-flanking scaffold86 junction | |||
| P13 | CTATCGTCTGACTCTGACTG | 49 | FFS1 flax genomic region |
| P14 | CAACGCCCACTCTCTTTCTTA | 52 | FFS2 flax genomic region |
| P15 | CCCTTAATTCTCCGCTCATGATCAG | 50 | RB region of TDNA |
| prb3 | CACTTCTTCAATTTTATTTCAATATGTCTTTC | 60 | Specific for FP967 TDNA insertion site on scaffold86 |
| prb5 | ATCAAACACTGATAGTTTAAACTGAAGGCGGG | 67 | Specific for RB region of TDNA |
| Event specific assay SAT2-LB: Detects internal inverted repeat of E. coli SpecR cassette adjacent to LB | |||
| P85 | TACATTAAAAACGTCCGCAATGTG | 59 | Located in LB |
| P86 | CCTGCTCAGGGATCACCGAA | 62 | In SAT of E.Coli SpecR cassette |
| prb28 | TATCCTGCCAAAAGCCGCGCCA | 68 | LB/Spec junction |