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. 2015 Apr 2;4:161. doi: 10.1186/s40064-015-0932-8

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© Donis-Maturano et al.; licensee Springer. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Death kinetics of macrophages and dendritic cells exposed to NETs from human blood neutrophils. Percentage of macrophages (A) or dendritic cells (B) stained with 7-Amino-Actinomycin-D (7AAD) after incubation with culture medium alone, with NETs or NETs supernatants. 7-AAD positive cells represent dead cells and were evaluated at 0.5, 3, 6, 12 and 24h post-stimulation. *P = 0.01; **P = 0.001; ***P < 0.0001, one-way ANOVA. Mfs: Macrophages, DC: Dendritic cells, NETs sn: NETs supernatants.