Figure 2.

NMN promotes Wallerian degeneration. (a) SCG neurites were cut and then left untreated or treated with 100 nM FK866 or with 100 nM FK866 and 1 mM NMN as shown and imaged at the indicated time points. (b) Degeneration index of SCG neurites treated as in panel (a), or with lower NMN concentrations, were calculated from three fields in three independent experiments (n=9, mean±S.E.M., one-way ANOVA with Bonferroni's post-hoc test, *P<0.05, ***P<0.001, ****P<0.0001, compared with untreated at the same time point). (c) Degeneration index of SCG neurites cut and then left untreated or treated with 100 nM FK866, 100 nM FK866 and 1 mM NMN or 100 nM FK866 and 1 mM NR as indicated and imaged at 0, 8 and 24 h were calculated in three fields of three independent experiments (n=9, mean±S.E.M., one-way ANOVA with Bonferroni's post-hoc test, ***P<0.001, ****P<0.0001, compared with untreated at the same time point). (d) Degeneration index of SCG neurites cut and then left untreated or treated as indicated was calculated in three fields of three (for NMN effect) or two (for NAD effect) independent experiments (n= 6–9, mean±S.E.M., one-way ANOVA with Bonferroni's post-hoc test, **P<0.01, ****P<0.0001, compared with untreated at the same time point). NS, not significant