Figure 1.

Identification of Tra2β target mRNAs by RNA immunoprecipitation and microarray analysis. (a) Lysates were prepared from HCT116 cells and subjected to IP assays with an anti-Tra2β antibody or normal rabbit IgG as described in Materials and Methods section. (b) The relative amount of Tra2β protein the anti-Tra2β IP or IgG IP materials was measured by western blotting. (c) mRNAs in the Tra2β IP materials were subjected to Ingenuity Pathway Analysis. The top 10-scored biological functions are listed. The level of significance was set at a P-value of 0.05 by the Fisher's exact test. (d) The abundance of mRNAs present in the Tra2β IP materials after the RIP assay was validated using qPCR with GAPDH and 18S as background controls. The values shown are the mean±S.D. (n=3). IP, immunoprecipitation; HC, heavy chain; LC, light chain