Table 2.
Molecular cytogenetic and immunohistochemical profile of liposarcomas with or without lipoma-like component
| Case | Histologic type | Fusion gene (RT-PCR) | DDIT3 break apart (FISH) | Karyotype | MDM2 | CDK4 | PPARγ | C/EBPα | Adipophilin | Perilipin | Ki-67 index |
|---|---|---|---|---|---|---|---|---|---|---|---|
| LC/MC | LC/MC | LC/MC | LC/MC | LC/MC | LC/MC | LC/MC | LC/MC | ||||
| 1 | MLSLC | FUS-DDIT3 (type II) | p/p | ND | 0/0 | 0/0 | 2+/3+ | 2+/3+ | 2+/2+ | 3+/2+ | 3.0/10.0 |
| 2 | MLSLC | FUS-DDIT3 (type I) | p/p | t(12;16), del(6), +8, −15, +mar | 0/0 | 0/0 | 3+/3+ | 3+/3+ | 1+/3+ | 3+/3+ | 3.4/11.8 |
| 3 | MLSLC | FUS-DDIT3 (type II) | p/p | ND | 0/0 | 0/0 | 1+/3+ | 1+/1+ | 1+/3+ | 3+/1+ | 2.2/17.0 |
| 4 | MLSLC | FUS-DDIT3 (type II) | p/p | ND | 0/0 | 0/0 | 2+/3+ | 2+/3+ | 1+/3+ | 3+/3+ | 3.6/10.6 |
| 5 | MLSLC | FUS-DDIT3 (type II) | p/p | ND | 0/0 | 0/0 | 2+/3+ | 2+/2+ | 1+/3+ | 3+/1+ | 0.8/7.4 |
| 6 | MLSLC | FUS-DDIT3 (type II) | p/p | t(12;16) | 0/0 | 0/0 | 3+/3+ | 2+/3+ | 2+/3+ | 3+/1+ | 2.4/12.2 |
| 7 | MLSLC | FUS-DDIT3 (type I) | p/p | ND | 0/0 | 0/0 | 2+/3+ | 3+/3+ | 1+/2+ | 3+/1+ | 3.0/8.6 |
| 8 | MLSLC | FUS-DDIT3(type II) | p/p | t(12;16) | 0/0 | 0/0 | 1+/3+ | 2+/3+ | 3+/3+ | 3+/1+ | 2.8/20.2 |
| 9 | MLS | FUS-DDIT3(type II) | ND | t(12;16), t(1;15) | NA/0 | NA/0 | NA/3+ | NA/2+ | NA/3+ | NA/2+ | NA/22.2 |
| 10 | MLS | FUS-DDIT3(type II) | ND | ND | NA/0 | NA/0 | NA/3+ | NA/2+ | NA/3+ | NA/1+ | NA/8.2 |
| 11 | MLS | FUS-DDIT3(type II) | ND | t(12;16) | NA/0 | NA/0 | NA/3+ | NA/2+ | NA/3+ | NA/3+ | NA/10.2 |
| 12 | MLS | FUS-DDIT3(type II) | NA/p | t(12;16), +8 | NA/0 | NA/0 | NA/3+ | NA/2+ | NA/3+ | NA/2+ | NA/6.4 |
| 13 | MLS | FUS-DDIT3(type II) | NA/p | t(12;16), t(1;8), add(9), +mar | NA/0 | NA/0 | NA/3+ | NA/3+ | NA/3+ | NA/3+ | NA/12.8 |
| 14 | MLS | FUS-DDIT3 (type I) | ND | t(12;16), +8, +9, +16, +add(16)t(12;16) | NA/0 | NA/0 | NA/3+ | NA/1+ | NA/3+ | NA/1+ | NA/11.4 |
| 15 | MLS | FUS-DDIT3(type II) | ND | t(12;16), +mar | NA/0 | NA/0 | NA/3+ | NA/3+ | NA/3+ | NA/2+ | NA/17.6 |
| 16 | MLS | FUS-DDIT3 (type I) | ND | t(12;16), add(6), −22 | NA/0 | NA/0 | NA/3+ | NA/2+ | NA/2+ | NA/1+ | NA/17.4 |
| 17 | MLS | FUS-DDIT3 (type I) | NA/p | ND | NA/0 | NA/0 | NA/3+ | NA/2+ | NA/3+ | NA/1+ | NA/18.4 |
| 18 | MLS | FUS-DDIT3(type II) | NA/p | ND | NA/0 | NA/0 | NA/3+ | NA/3+ | NA/3+ | NA/1+ | NA/15.6 |
| 19 | MLS | FUS-DDIT3(type II) | ND | t(12;16), +8 | NA/0 | NA/0 | NA/3+ | NA/3+ | NA/3+ | NA/1+ | NA/18.4 |
| 20 | WDLS | n | Uncountable | +1 ~ 3 ring | 1+/2+ | 2+/3+ | 1+/2+ | 1+/2+ | 1+/1+ | 3+/1+ | 1.8/4.8 |
| 21 | WDLS | n | n/n | +1 ~ 2 ring, +mar | 1+/2+ | 3+/3+ | 3+/3+ | 2+/2+ | 1+/1+ | 3+/1+ | 4.8/16.0 |
| 22 | WDLS | ND | n/n | ND | 2+/3+ | 3+/2+ | 3+/2+ | 1+/1+ | 1+/1+ | 3+/0 | 2.8/5.8 |
| 23 | WDLS | n | Uncountable | +Ring | 1+/1+ | 2+/1+ | 2+/1+ | 2+/1+ | 1+/1+ | 3+/0 | 1.8/3.4 |
| 24 | DDLS | ND | Uncountable | +Giant, +ring | 2+/2+ | 2+/3+ | 1+/2+ | 1+/2+ | 1+/1+ | 3+/0 | 1.6/10.4 |
| 25 | DDLS | n | Uncountable | 108–115, complex | 1+/3+ | 2+/3+ | 1+/2+ | ND | 1+/2+ | 3+/0 | 2.8/32.4 |
| 26 | DDLS | ND | ND | ND | 0/2+ | 2+/3+ | 2+/3+ | 2+/2+ | 1+/1+ | 3+/1+ | 0.8/5.2 |
| 27 | DDLS | ND | n/n | ND | 1+/3+ | 1+/3+ | 1+/3+ | 1+/2+ | 1+/1+ | 3+/0 | 5.6/23.0 |
| 28 | DDLS | n | Uncountable | ND | 1+/2+ | 1+/3+ | 2+/3+ | 1+/2+ | 1+/3+ | 2+/0 | 1.0/24.0 |
| 29 | DDLS | n | NA/n | ND | NA/2+ | NA/3+ | NA/2+ | NA/2+ | NA/0 | NA/0 | NA/35.0 |
DDLS dedifferentiated liposarcoma, Ki-67 index percentage of positive nuclei/500 cells, LC lipoma-like component, MC myxoid component, n negative, MLS myxoid liposarcoma, MLSLC MLS with lipoma-like component, NA not available, ND not done, p positive, WDLS well-differentiated liposarcoma; −, 0 %; 1+, 1–25 %; 2+, 26–50 %; 3+, >50 %