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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Nat Med. 2015 Feb 16;21(3):248–255. doi: 10.1038/nm.3806

Figure 3. MCC950 blocks NLRP3 dependent ASC oligomerization.

Figure 3

(a) Western blots of cell lysates, supernatants and cross-linked cytosolic pellets from BMDM stimulated with LPS and nigericin and treated with MCC950 and parthenolide. These results are representative of three independent experiments. (b) Live cell imaging of ASC-cerulean cells treated with MCC950 and stimulated with ATP. At least five different images were taken of each condition at an original magnification of ×40. Results are representative of three independent experiments. (c) The percentage of ASC-cerulean cells containing an ASC speck after treatment with MCC950 (0.05-10 µM) and stimulation with nigericin, LeuLeu-Ome or lethal toxin. Data shown represent the mean ± S.D. from triplicate measurements.