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. 2015 Feb 19;290(15):9348–9361. doi: 10.1074/jbc.M113.510495

FIGURE 8.

FIGURE 8.

Knockdowns of p22phox-dependent NOX isoforms (NOX1, -2, and -4) cause a decrease in endogenous H2O2 and DNA damage in 32D/FLT3-ITD expressing cells. In A-C, Western blot analysis of the NOX4 (A), NOX1 (B), and NOX2 (C) proteins levels in 32D/FLT3-WT and 32D/FLT3-ITD cells. β-Actin was used as a loading control. D–F, at 24 h, Western blot analysis of the NOX4 (D), NOX1 (E), and NOX2 (F) proteins levels following the siRNA transfection. GAPDH was used as a loading control. G, flow cytometric analysis of H2O2 levels following the NOX siRNA knockdowns, using PO1 probe, compared with negative siRNA control (control). H, flow cytometric analysis of γ-H2AX levels following the NOX siRNA knockdowns, compared with negative siRNA control (control). The mean is representative of three independent experiments. The asterisk indicates a statistically significant difference (p < 0.05) as analyzed by Student's t test. The error bars represent ± S.D.