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. 2015 Feb 24;290(15):9442–9454. doi: 10.1074/jbc.M115.636803

FIGURE 3.

FIGURE 3.

HP-β-CD and biocleavable polyrotaxanes had opposite effects on the levels of LC3-II and p62. A, immunoblot analysis for LC3, p62, Beclin-1, and β-actin in the normal and NPC1 fibroblasts treated with Baf A (400 nm), HP-β-CD, and HEE-SS-PRX at various concentrations for 24 h. B, relative protein level for LC3-II/β-actin (gray bars) and p62/β-actin (black bars) as determined by the band intensities of the immunoblot. The intensity of each band was normalized with β-actin (*, p < 0.05; **, p < 0.01; ****, p < 0.001). C, immunoblot analysis for LC3, p62, and β-actin in the normal fibroblasts treated with Baf A (400 nm), HP-β-CD, and HEE-SS-PRX at various concentrations for 24 h. D, immunoblot analysis for LC3, p62, and β-actin in the NPC1 fibroblasts treated with HP-β-CD and HEE-SS-PRX in the presence of Baf A (400 nm) for 24 h. The values are expressed as the mean ± S.D. (error bars) (n = 3).