FIGURE 5.

Biocleavable polyrotaxanes improved impaired autophagic flux in NPC1 fibroblasts. A, CLSM images of normal and NPC1 fibroblasts transiently expressing mRFP-GFP-LC3 (green and red puncta indicate GFP and mRFP, respectively) treated with HP-β-CD (10 mm) and HEE-SS-PRX (1 mm β-CD) for 24 h (scale bars, 20 μm). B, the numbers of mRFP⁺-GFP⁺ and mRFP⁺-GFP⁻ puncta in the normal and NPC1 fibroblasts expressing mRFP-GFP-LC3. The values are expressed as the mean ± S.E. (error bars) of 30 cells (*, p < 0.05; **, p < 0.01; ***, p < 0.005).