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. 2015 Feb 24;290(15):9442–9454. doi: 10.1074/jbc.M115.636803

FIGURE 6.

FIGURE 6.

Biocleavable polyrotaxanes do not affect lysosomal enzymatic activity. A, CLSM images of the activity of cathepsin B in the normal and NPC1 fibroblasts treated with Baf A (400 nm), HP-β-CD (10 mm), and HEE-SS-PRX (1 mm) for 24 h, as determined by Magic Red cathepsin B (scale bars, 50 μm). B, relative fluorescence intensities of the Magic Red cathepsin B. The values are normalized with the untreated normal fibroblasts (n = 3) (***, p < 0.005; NS, not significant). C, immunoblot analysis for pro- and mature cathepsin B and β-actin in the normal and NPC1 fibroblasts treated with Baf A (400 nm), HP-β-CD (10 mm), and HEE-SS-PRX (1 mm β-CD) for 24 h. D, relative band intensity ratios of mature cathepsin B/pro-cathepsin B. The values are normalized with the untreated normal fibroblasts and expressed as the mean ± S.D. (error bars) (n = 3) (*, p < 0.05; NS, not significant).