TABLE 2.
GBS strains used in this work
| Strain name | Description | Mutated protein (full-length aa) |
|---|---|---|
| GBS 515 | Wild type strain | |
| ΔcpsE | cpsE deletion | Details in Ref. 14 |
| ΔcpsA | cpsA deletion | Deletion of aa 11–452 (458 aa) |
| CpsA(Δext) | Deletion of the CpsA extracellular domain | Deletion of aa 96–458 (458 aa) |
| CpsA(ΔLytR) | Deletion of the CpsA LytR domain | Deletion of aa 236–458 (458 aa) |
| ΔcpsB | cpsB deletion | Deletion of aa 4–240 (243 aa) |
| CpsB(R139A) | Point mutation in the phosphatase active site | Arginine to alanine in position 139 (243 aa) |
| CpsB(R206A) | Point mutation in the phosphatase active site | Arginine to alanine in position 206 (243 aa) |
| ΔcpsC | cpsC deletion | Deletion of aa 1–222 (230 aa) |
| CpsC(ΔC-term) | Deletion of the CpsC intracellular C-terminal portion | Deletion of aa 198–230 (230 aa) |
| CpsC(Δext) | Deletion of the CpsC extracellular domain | Deletion of aa 53–153 (230 aa) |
| ΔcpsD | cpsD deletion | Deletion of aa 11–225 (232 aa) |
| CpsD(K49A) | Point mutation in the autokinase active site | Lysine to alanine in position 49 (232 aa) |
| CpsD(ΔP-Tyr) | Phosphoacceptor site C-terminal deletion | Deletion of aa 213–224 (232 aa) |