TABLE 3.
Biochemical characterization of the purified CPS from selected mutant strains
Quantification of the CPS purified from bacterial pellets and from spent growth media from 1-liter cultures. The average molecular mass of CPS was estimated by HPLC-SEC. The mass range represents 95% of the area of the molecular size distribution. NA, not available.
| Strain | Fraction | CPS | Mass | Mass range |
|---|---|---|---|---|
| mg | kDa | kDa | ||
| 515 | Bacteria | 4.1 | 167 | 49–616 |
| Medium | 0.5 | NA | NA | |
| ΔcpsA | Bacteria | 2.8 | 237 | 62–1075 |
| Medium | 6.7 | >1,330 | 115 to >1,330 | |
| CpsC(Δext) | Bacteria | 3.3 | 55 | 23–136 |
| CpsD(K49A) | Bacteria | 1.7 | >1,330 | 1013 to >1,330 |
| Medium | 5.6 | >1,330 | >1,330 |