FIGURE 3.

Panel of G proteins activated by CCR5. Real time measurement of BRET signal in HEK293T cells coexpressing CCR5 and G protein biosensors (black bars) or G protein biosensors only (open bars) and stimulated for 1 min with 100 nm of the chemokines CCL3, CCL4, CCL5, CCL7, CCL8, and CCL13 or 1 μm of the small molecules TAK779 (T) and Maraviroc (M) is shown. The results are expressed as the difference in BRET signal measured in the presence and absence of stimulation. The data represent the means ± S.E. of at least six independent experiments. Statistical significance between cells expressing or not CCR5. (***, p < 0.001; **, p < 0.01; *, p < 0.1) and between chemokines (###, p < 0.001; ##, p < 0.01; #, p < 0.1) was assessed using Tukey's test.