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Activation of Gi1, Gob, and G12 by CCR5. Real time measurement of BRET signal in HEK293T cells coexpressing CCR5 and G protein biosensors and stimulated with increasing concentration of chemokines is shown. The results are expressed as the differences in BRET signal measured in the presence and absence of chemokines. The data represent the means ± S.E. of three independent experiments.
