TABLE 2.

Binding and signaling parameters of CCR5

Binding and functional parameters were measured on HEK293 or CHO-K1 cells expressing CCR5. The pIC₅₀, pEC_50, and E_max values were obtained from experiments as displayed in Figs. 2, 4, 5, and 9. The values represent the means ± S.E. of at least three independent experiments. ND, not determined.

Ligands	[125I]CCL4		Gαi1		Gαob		Gα12		β-Arr2		cAMP		Ca2+
	pIC50	pKi	pEC50	Emax	pEC50	Emax	pEC50	Emax	pEC50	Emax	pEC50	Emax	pEC50	Emax
CCL3	−8.7 ± 0.1	−8.9 ± 0.1	−8.1 ± 0.4	−0.09 ± 0.02	−8.1 ± 0.4	−0.08 ± 0.02	−8.6 ± 0.3	−0.017 ± 0.002	−8.6 ± 0.2	0.08 ± 0.01	−10.6 ± 0.2	43 ± 3	−7.2 ± 0.1	119 ± 12
CCL4	−9.2 ± 0.1	−9.4 ± 0.1	−8.5 ± 0.2	−0.07 ± 0.02	−8.3 ± 0.2	−0.10 ± 0.01	−8.1 ± 0.2	−0.016 ± 0.002	−8.1 ± 0.1	0.10 ± 0.01	−9.1 ± 0.1	44 ± 3	−8.2 ± 0.2	75 ± 5
CCL5	−8.2 ± 0.1	−8.4 ± 0.1	−9.1 ± 0.5	−0.09 ± 0.02	−9.2 ± 0.1	−0.10 ± 0.01	−9.0 ± 0.3	−0.020 ± 0.001	−8.1 ± 0.2	0.10 ± 0.01	−10.3 ± 0.3	34 ± 2	−8.0 ± 0.2	91 ± 3
CCL8	−8.2 ± 0.1	−8.4 ± 0.1	−8.2 ± 0.1	−0.09 ± 0.01	−8.1 ± 0.3	−0.06 ± 0.01	−8.1 ± 0.4	−0.015 ± 0.003	−7.0 ± 0.2	0.08 ± 0.01	−9.2 ± 0.2	39 ± 3	−7.4 ± 0.1	89 ± 3
CCL13	−7.2 ± 0.1	−7.4 ± 0.1	ND	ND	−7.6 ± 0.1	−0.05 ± 0.01	ND	ND	ND	ND	ND	ND	ND	ND