TABLE 3.

Binding and signaling parameters of CCR2

Binding and functional parameters were measured on HEK293 or CHO-K1 cells expressing CCR2. The pIC₅₀, pEC₅₀, and E_max values were obtained from experiments as displayed in Figs. 2, 7, 8, and 9. The values represent the means ± S.E. of at least three independent experiments. ND, not determined.

Ligands	[125I]CCL2		Gαi1		Gαob		Gα12		β-Arr2		cAMP		Ca2+
	pIC50	pKi	pEC50	Emax	pEC50	Emax	pEC50	Emax	pEC50	Emax	pEC50	Emax	pEC50	Emax
CCL2	−9.6 ± 0.1	−10.0 ± 0.1	−8.7 ± 0.2	−0.13 ± 0.01	−8.0 ± 0.1	−0.15 ± 0.01	−8.4 ± 0.4	−0.042 ± 0.004	−8.5 ± 0.1	0.14 ± 0.01	−9.1 ± 0.3	31 ± 4	−8.2 ± 0.1	86 ± 5
CCL7	−9.2 ± 0.1	−9.6 ± 0.1	−8.4 ± 0.2	−0.10 ± 0.01	−8.2 ± 0.1	−0.12 ± 0.01	−8.2 ± 0.2	−0.030 ± 0.003	−7.7 ± 0.1	0.12 ± 0.01	−9.3 ± 0.2	29 ± 4	−7.5 ± 0.2	86 ± 8
CCL8	−8.5 ± 0.1	−8.9 ± 0.1	−8.5 ± 0.2	−0.06 ± 0.01	−8.8 ± 0.4	−0.05 ± 0.01	ND	ND	−7.7 ± 0.1	0.03 ± 0.01	−8.4 ± 0.2	31 ± 4	ND	ND
CCL13	−7.9 ± 0.1	−8.3 ± 0.1	−8.5 ± 0.4	−0.06 ± 0.01	−7.9 ± 0.2	−0.08 ± 0.01	ND	ND	−7.0 ± 0.1	0.14 ± 0.01	ND	ND	ND	ND