FIGURE 9.

Down-regulation of cell surface GRP78 by RNAi suppresses α₂M*-induced expression of ATP citrate lyase (black bars) and fatty-acid synthase (white bars) in 1-LN (A) and DU-145 (B) prostate cancer cells. The panels in both immunoblots are as follows: 1, Lipofectamine + α₂M*; 2, GRP78 dsRNA (100 nm/48 h)/Lipofectamine and then α₂M*; and 3, scrambled dsRNA (100 nm/48 h)/Lipofectamine and then α₂M*. The changes in expression of target proteins are in arbitrary units and are the mean ± S.E. from four experiments. Values significantly different at the 5% level for GRP78 dsRNA-transfected cells compared with the controls are denoted by an asterisk. A representative immunoblot of fatty-acid synthase and ATP citrate lyase in 1-LN (A) and DU-145 (B) cells from four experiments is shown below the respective bar diagrams.