FIGURE 7.

Prediction of histone binding sites onto the PRMT5-MEP50 complex. A, the predicted interacting residues on the cross-dimer pair of XlPRMT5-MEP50 was determined using the SPPIDER and PredUs algorithms and mapped onto the structure, as shown in yellow. Shown are docking of H2A-H2B dimer (orange/yellow) (B) and H3-H4 dimer (blue/green) (C) onto XlPRMT5-MEP50 (PRMT5 monomer (purple), its directly bound MEP50 molecule (pink), and the cross-dimer MEP50 (blue)), using ClusPro with attractive forces as determined by the peptide array and predictions in A.