TABLE 3.

The effect of mutagenesis of Tyr-33 on kinetics of transport by Glt_Ph

Shown are kinetic parameters for mutant Glt_Ph reconstituted in liposomes composed of a 3:1 mixture of E. coli polar lipid extract:trimethyl PE. Data were derived by fitting the l-aspartate concentration-response data to the Michaelis-Menten equation. The values are averaged from experiments performed in triplicate, and errors indicate S.E. No significant difference was observed for K_0.5 values.

	WT	Y33F	Y33S	Y33W	Y35F	Y35S
Aspartate K0.5 (nm)	84.7 ± 8.6	86.3 ± 13.0	116.0 ± 19.8	61.0 ± 9.0	57.5 ± 9.5	57.7 ± 10.6