FIGURE 3.

Stabilities of the hexameric structures of D1 and D2 dimers. We analyzed 1.73 μm (as hexamers) wild-type (upper traces), D1 dimer (middle traces), and D2 dimer (bottom traces) TClpB by HPLC size exclusion chromatography at 55 °C in the presence of 2 mm ATP and 150 (dashed line), 500 (thin solid line), or 800 (thick solid line) mm KCl. The arrows indicate the calculated retention time corresponding to 577, 385, and 192 kDa (hexamers, tetramers, and dimers of the 96.2 kDa TClpB subunit, respectively).