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. 2015 Feb 24;290(15):9812–9822. doi: 10.1074/jbc.M114.616219

FIGURE 6.

FIGURE 6.

β Cell-specific deletion of Set7/9 in mice. A, schematic of WT and mutant alleles present in control and mutant mice, respectively, showing positions of the exons (numbered) and the Loxp sites. B, Immunoblot analysis of whole cell lysates of islets isolated from Setd7+/+;MIP1-CreERT (MIP1-Cre), Setd7Loxp/Loxp (Lox/Lox), and MIP1-CreERT;Setd7Loxp/Loxp (SetΔβ) mice for Set7/9 (top panel) and GAPDH (bottom panel). C, results of intraperitoneal glucose tolerance test on MIP1-Cre (n = 12), Lox/Lox (n = 9), and SetΔβ (n = 12) mice (left panel) with a corresponding area under the curve (AUC) analysis (right panel). *, p < 0.05 compared with either MIP1-Cre or Lox/Lox. D, results of glucose-stimulated insulin release assays from islets isolated from MIP1-Cre, Lox/Lox, and SetΔβ mice (left panel), and the corresponding ratio of insulin secreted at high glucose relative to low glucose is shown in the right panel. n = 3, *, p < 0.05 compared with either MIP1-Cre or Lox/Lox. E, results of real-time RT-PCR for the indicated genes from islets isolated from control (MIP1-Cre and Lox/Lox) versus SetΔβ mice. Data are normalized to Actb mRNA levels; n = 4 independent islet isolations; *, p < 0.05 compared with control.